Assignment: Process RNA-seq files
DUE: Friday 7/31 by 9AM
Using the count files you created for your two different tissues, you’ll find the differences between the tissues. Everything should be in/backed up to the same GitHub repository as your processing code.
- Analyze the RNA-seq. You need to have an
.Rmd
file showing all your code and it must include:- PCA
- differential expression table
- volcano plot and/or MA plot
- pathway analysis
- 10 minute presentation for Friday at 12PM explaining your figures/results. The presentation should include:
- Any figures you made during the course of the analysis (PCA, volcano plot, MA plot, pathway visualization)
- Anything interesting you want to mention about the top genes or pathways